NEET Biology 2020 - Biotechnology: Principles & Processes

Question - 1

What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis?

  • A The larger the fragment size, the farther it moves
  • B The smaller the fragment size, the farther it moves
  • C Positively charged fragments move to farther end
  • D Negatively charged fragments do not move

Question - 2

A gene whose expression helps to identify transformed cell is known as :

  • A Selectable marker
  • B Vector
  • C Plasmid
  • D Structural gene

Question - 3

The DNA fragments separated on an agarose gel can be visualised after staining with :

  • A Bromophenol blue
  • B Acetocarmine
  • C Aniline blue
  • D Ethidium bromide

Question - 4

Which of the following restriction enzymes produces blunt ends?

  • A Sal I
  • B Eeo RV
  • C Xho I
  • D Hind III

Question - 5

A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using:

  • A Eeo RI
  • B Taq polymerase
  • C Polymerase III
  • D Ligase

Question - 6

Which of the following is not a feature of the plasmid?

  • A Single stranded
  • B Independent replication
  • C Circular structure
  • D Small, circular double-stranded

Question - 7

Commonly used vectors for human genome sequencing are:

  • A T-DNA
  • B BAC vectors
  • C Expression vector
  • D TIC cloning vector

Question - 8

DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by:

  • A Centrifugation
  • B Polymerase Chain Reaction
  • C Electrophoresis
  • D Restriction mapping

Question - 9

Biolistics (Gene gun) is suitable for:

  • A Disarming pathogen vectors
  • B Transformation of plant cells
  • C Constructing recombinant DNA by joining the vectors
  • D DNA fingerprinting

Question - 10

In genetic engineering, the antibiotics are used.

  • A As selectable markers
  • B To select healthy vectors
  • C As sequences from where replication starts
  • D To keep the culture free ofinfection
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