Biology - Biotechnology : Principles and Processes
Exam Duration: 45 Mins Total Questions : 30
Maximum number of bases in plasmids discovered so far is
- (a)
50 KILOBASE
- (b)
5000 KILOBASE
- (c)
500 KILOBASE
- (d)
5 KILOBASE
The technique of genetic engineering does not include.
- (a)
Gene cloning
- (b)
Gene transfer
- (c)
Vector cloning
- (d)
Host cloning
The plasmids are the
- (a)
The enzymes present in the prokaryotes
- (b)
Lysozymes in eukaryotes
- (c)
Extra chromosomal DNA in bacteria
- (d)
Mitochondrial DNA
The purpose of the presence of restriction enzymes in bacteria is
- (a)
Gene cloning
- (b)
Defence against infecting virus
- (c)
Gene transfer
- (d)
Genetic recombination
DNA ligase helps in joining the DNA fragments by forming
- (a)
Hydrogen bonds
- (b)
Phosphodiester bonds
- (c)
Covalent bonds
- (d)
None of the above
In Blue - white selection,
- (a)
The selection of recombinants is easier based on colour reaction
- (b)
It avoids the simultaneous plating of two plates having different antibiotics
- (c)
It is based on the insertional inactivation of lac Z gene present on the vector
- (d)
White coloured colonies are the non recombinant DNA molecules
DNA fragments generated by the restriction endonucleases in a chemical reaction is separated by
- (a)
Centrifugation
- (b)
Polymerase chain reaction
- (c)
Electrophoresis
- (d)
Restriction mapping
The tools of genetic engineering are the following except
- (a)
Cosmid
- (b)
Ti plasmid
- (c)
Endonucleases
- (d)
Helicase
The restriction endonucleases of a cell do not cleave its own DNA because of
- (a)
Difference in recognition sequence
- (b)
Methylation of A or C at the recognition site
- (c)
Restriction endonucleases cleave the DNA out of the cells
- (d)
None of the above
In genetic engineering which of the following is used?
- (a)
Plasmid
- (b)
Plastid
- (c)
Mitochondria reticulum
- (d)
Endoplasmic
Restriction enzymes
- (a)
Are endonucleases which cleave DNA at specific sites
- (b)
Make DNA complementary to an existing DNA or RNA
- (c)
Cut or join DNA fragments
- (d)
Are required in vector less direct gene transfer
When exonuclease is added to the tube containing recombinant DNA,
- (a)
The recombinant DNA will be digested into nucleotides
- (b)
The recombinant DNA will be cleaved at specific sites
- (c)
The recombinant DNA will be retransformed
- (d)
Nothing will happen
Introduction of foreign genes for improving genotype is called
- (a)
Biotechnology
- (b)
Tissue culture
- (c)
Genetic engineering
- (d)
Both (1) & (3)
The linking of antibiotic resistance gene with plasmid vector became possible with
- (a)
DNA polymerase
- (b)
Exonuclease
- (c)
DNA ligase
- (d)
Endonuclease
In recombinant DNA technology, a plasmid vector is cleaved by
- (a)
Modified DNA ligase
- (b)
A heated alkaline solution
- (c)
The same enzyme that cleaves donor DNA
- (d)
Different enzyme that cleaved the donor DNA
Plasmids are important in biotechnology because they contain
- (a)
Recognition sites on recombinant DNA strands
- (b)
Provirus incorporated into the host DNA
- (c)
A vehicle for insertion of recombinant DNA into bacteria
- (d)
Surface for respiratory process in bacteria
Telomeric sequence, centromere, autonomously replicating sequence from yeast are characteristic features of
- (a)
YAC
- (b)
BAC
- (c)
Retrovirus
- (d)
Both (1), (2) & (3)
PCR technique is best for
- (a)
DNA synthesis
- (b)
Protein amplification
- (c)
DNA amplification
- (d)
DNA ligation
Which of the following is not an application of PCR?
- (a)
ELISA
- (b)
Diagnosis of pathogens
- (c)
DNA fingerprinting
- (d)
In palaeontology
The optimum temperature for polymerisation in PCR is ____________ while the enzyme responsible for the mentioned step can tolerate temperatures upto __________. Select the correct option according to the blanks.
- (a)
950C, 600 C
- (b)
940C, 950C
- (c)
720C, 950C
- (d)
950C, 720C
In case of Bam HI, H represents
- (a)
Genus
- (b)
Species
- (c)
Name of scientist
- (d)
Strain
A tumor inducing plasmid widely used in the production of transgenic plants is that of
- (a)
Escherichia coli
- (b)
Bacillus thuringiensis
- (c)
Staphylococcus aureus
- (d)
Agrobacterium tumefaciens
An antibiotic resistance gene in a vector helps in selection of
- (a)
Competent cells
- (b)
Transformed cells
- (c)
Recombinant cells
- (d)
Both (2)&(3)
'Disarmed' in disarmed vector represents
- (a)
Removal of T-DNA from Ti plasmid
- (b)
Insertional inactivation of β-galactosidase gene
- (c)
Insertional inactivation of antibiotic gene
- (d)
Both (2) & (3)
For transformation, micro-particles coated with DNA to be bombarded with gene gun are made up of
- (a)
Silicon or Platinum
- (b)
Gold or Tungsten
- (c)
Silver or Platinum
- (d)
Platinum or Zinc
Stirred-tank bioreactors have been designed for
- (a)
Availability of oxygen throughout the process
- (b)
Addition of preservatives to the product
- (c)
Purification of the product
- (d)
Ensuring anaerobic conditions in the culture vessel
Which of the following are used in gene cloning?
- (a)
Nucleotides
- (b)
Lomasomes
- (c)
Mesosomes
- (d)
Plasmids
In genetic engineering, a DNA segment (gene) of interest, is transferred to the host cell through a vector. Consider the following four agents (A-D) in this regard and select the correct option about which one or more of these can be used as a vector/vectors
Statements
(A) A bacterium (B) Plasmid
(C) Plasmodium (D) Bacteriophage
- (a)
(A), (B) & (D) only
- (b)
(A) only
- (c)
(A) & (C) only
- (d)
(B) & (D) only
In plant biotechnology, PEG is used in
- (a)
Protoplast isolation
- (b)
Cell culture preparation
- (c)
Protoplast fusion
- (d)
Hardening
Which of the following is considered as molecular glue?
- (a)
Alkaline phosphate
- (b)
Restriction endonuclease
- (c)
DNA ligase
- (d)
DNA polymerase