Biology - Biotechnology and its Applications
Exam Duration: 45 Mins Total Questions : 30
Electroporation is used for
- (a)
increasing uptake of plasmids by bacteria or animal cells
- (b)
DNA replication
- (c)
RNA replication
- (d)
to carry DNA into plant cells
Which enzyme is useful in genetic engineering?
- (a)
DNAase
- (b)
Amylase
- (c)
Lipase
- (d)
Restriction endonuclease
Match the following columns.
Column I | Column II | ||
---|---|---|---|
A | Pasteur | 1 | Small glycoproteins |
B | Polio vaccine | 2 | Rabies vaccine |
C | Humulin | 3 | Robert Brown |
D | Interferons | 4 | Johnes Salk |
5 | Eli Lilly |
- (a)
A B C D 2 3 1 5 - (b)
A B C D 5 2 1 4 - (c)
A B C D 4 1 2 3 - (d)
A B C D 2 4 5 1
Which one is a true statement regarding DNA polymerase used in PCR?
- (a)
It is used to ligate introduced DNA in recipient cells
- (b)
It serves as a selectable marker
- (c)
It is isolated from a virus
- (d)
It remains active at high temperature
Wildlife is
- (a)
all biota excluding man, domestic animlas and cultivated crops
- (b)
all vertebrates of reserve forests
- (c)
all animals of reserve forests
- (d)
all animals and plant of reserve forest
Bandipur (Karnataka) National park is site if
- (a)
Deer project
- (b)
Peacock project
- (c)
Elephant project
- (d)
Tiger project
'Red Data Book' is produced by
- (a)
IBWL
- (b)
ZSI
- (c)
WWF
- (d)
IUCN
Bt toxin does not affect
- (a)
Army worm
- (b)
Bees
- (c)
Beetles
- (d)
Mosquitoes
Which one of the pair is not the use of GM crops?
- (a)
Pest-resistant - Bt cotton
- (b)
Golden rice - enriched vitamin A
- (c)
Post-harvest loss reduction - flavr - savr variety of tomato
- (d)
Terminator gene - Bt cotton
In bacteria, plasmid is
- (a)
extrachromosomal material
- (b)
main DNA
- (c)
non-functional DNA
- (d)
repetitive gene
A restriction endonuclease breaks bonds between the
- (a)
base pairs of a DNA molecule
- (b)
base pairs of a DNA-RNA hybrid molecule
- (c)
sugar and phosphate components of a nucleic acid molecule
- (d)
exons and introns of a DNA molecule.
Using recombinant DNA technology, genes from a donor cell can be inserted into a bacterium for DNA replication and protein synthesis. The kind of cells that can be used as gene donors in this technology are
- (a)
bacteria only
- (b)
either yeast or bacteria
- (c)
eukaryotic cells only
- (d)
any of these.
Fill up the blanks and select the correct option.
(i) EcoRI cuts the DNA between bases ________ only when the sequence __________ is present in the DNA duplex.
(ii) Disruption of the cell membranescan be achieved by treating the bacterial cells,plant cellsand fungal cells with enzymesrespectively ________ and _________ .
(iii) Since DNA has a ________ charge, it moves towards the ___________ of the electrophoretic chamber.
- (a)
(i) G and A, GAATTC (ii) endonuclease, cellulase, chitinase (iii) negative, anode
- (b)
(i) G and A, GAATTC (ii) lysozyme, cellulase, chitinase (iii) positive, cathode
- (c)
(i) G and A, GAATTC (ii) lysozyme, cellulase, chitinase (iii) negative, anode
- (d)
(i) G and A, GAAATC (ii) lysozyme, cellulase, chitinase (ili) positive, cathode
First genetically modified plant commercially released in India is
- (a)
golden rice
- (b)
Flavr Savr
- (c)
Bt brinjal
- (d)
Bt cotton
________ is a single stranded DNA or RNA, tagged with a radioactive molecule and is used to detect mutated genes.
- (a)
RNAi
- (b)
Probe
- (c)
Plasmid
- (d)
Primer
You discovered a novel eukaryotic organism that glows in the dark. You believe this trait is due to a single gene, and you wish to clone the gene. Which of the following strategies is most likely to be successful?
- (a)
Isolate the genomic DNA from the organism, digest with a restriction endonuclease, insert into a plasmid vector and transform into bacteria. Screen colonies for the ability to glow in the dark
- (b)
Isolate the genomic DNA from the organism, digest with a restriction endonuclease, insert into a plasmid vector and transform into eukaryotic cells such as yeast. Screen colonies for the ability to glow in the dark
- (c)
Isolate mRNA from the organism, reverse transcribe and generate cDNA, insert into a plasmid vector and transform into bacteria. Screen colonies for the ability to glow in the dark
- (d)
Isolate mRNA from the organism, reverse transcribe and generate cDNA, insert into a plasmid vector and transform into eukaryotic cells such as yeast. Screen colonies for the ability to glow in the dark
A protoxin is
- (a)
a primitive toxin
- (b)
a denatured toxin
- (c)
toxin produced by protozoa
- (d)
inactive toxin
Assertion : Restriction enzymes Hin and Hpa are produced from two different genera of bacteria.
Reason: Hin is produced from Haemophilus while Hpa is produced from Hematococcus.
- (a)
If both assertionand reason are true and reason is the correct explanation of assertion.
- (b)
If both assertionand reason are true but reason is not the correct explanation of assertion.
- (c)
If assertionis true but reason is false.
- (d)
If both assertionand reason are false.
Assertion: The first clinical gene for ADA therapy was given to cure SCID.
Reason: The normal gene was delivered into the patient's cells using retroviral vector.
- (a)
If both assertion and reason are true and reason is the correct explanation of assertion
- (b)
If both assertion and reason are true but reason is not the correct explanation of assertion
- (c)
If assertion is true but reason is false
- (d)
If both assertion and reason are false
Which struture involved in genetic engineerting:
- (a)
Plastid
- (b)
Plasmid
- (c)
Codon
- (d)
None
Genetic engineering aims at :
- (a)
Destroying wild gene
- (b)
Preserving defective gene
- (c)
Curing human disease by introducing new gene
- (d)
All the above
Thermal cycle takes place in which technique
- (a)
Gel electrophoresis
- (b)
PCR- techinque
- (c)
Centrifugation
- (d)
Southern blotting
According to EFB, "The integration of natural science and organisms, cells, parts thereof and molecular analogues for products and services,"is known as-
- (a)
Biochemistry
- (b)
Bioinformatics
- (c)
Biotechnology
- (d)
Biology
Cry - gene which synthesize crystal protein isolated from:
- (a)
Bacillus thuriengensis
- (b)
Rhizbium
- (c)
Bacillus polymyxa
- (d)
Colostridium
The C - preptide is
- (a)
not present in proinsulin
- (b)
present in mature insulin
- (c)
removed during maturation of insulin
- (d)
also present in artificial insulin
Which kind of therapy was given in 1990 to a four year old girl with adenosine deaminase (ADA) deficiency?
- (a)
Immunotherapy
- (b)
Radiation therapy
- (c)
Gene therapy
- (d)
Chemotherapy
Trangenic Brassica napus has been used for synthesis of
- (a)
Hirudin
- (b)
Heparin
- (c)
Polygalacturonase
- (d)
Cry protein
According to the latest estimates how many documented varieties of basmati rice are grown in India?
- (a)
30
- (b)
27
- (c)
118
- (d)
42
The use of bioresources by multinational companies and other organisations without proper authorisation from the countries and people concerned without compensatory payment is called
- (a)
Bioethics
- (b)
Biopiracy
- (c)
Bioterror
- (d)
Bioweapon
Choose the incorrect statement w.r.t. Ethical issues in biotechnology
- (a)
Basmati rice is distinct for its unique aroma and flavour and 27 documented varieties of Basmati are grown in India
- (b)
In 1997, an american company got patent rights on Basmati rice through the US patent and Trademark office.
- (c)
Biopiracy is the form used to refer to the use of bioresources by multinational companies without proper authorisation from the countries and people concered without compensatory payment
- (d)
The current interest in the manipulation of microbes, plants and animals has raised no ethical questions.