Biology - Biotechnology : Principles and Processes
Exam Duration: 45 Mins Total Questions : 30
Recombinant DNA is obtained by cleaving the pro DNA by
- (a)
Restriction endonucleases
- (b)
Exonucleases
- (c)
Ligases
- (d)
Primase
The first recombinant DNA was constructed by
- (a)
Kary Mullis from Salmonella
- (b)
Kary Mullis from Escherichia
- (c)
Cohen & Boyer from Salmonella
- (d)
Cohen & Boyer from Escherichia
The purpose .of the presence of restriction enzymes is
- (a)
Restricting the multiplication of the bacteria
- (b)
Restricting the multiplication of the virus
- (c)
Restricting the growth of the bacteriophage in bacterium
- (d)
Restricting the growth and multiplication of bacterium
Many restriction enzymes cleave both strands of DNA
- (a)
Typically in the centre of the recognition sequence
- (b)
Typically in the recognition sequence asymmetrically
- (c)
Typically in the recognition sequence symmetrically
- (d)
Typically in the methylated sequence asymmetrically
DNA ligase helps in joining the DNA fragments by forming
- (a)
Hydrogen bonds
- (b)
Phosphodiester bonds
- (c)
Covalent bonds
- (d)
None of the above
The purpose of using alkaline phosphatase in RDNA technology is
- (a)
To Provide energy
- (b)
To provide phosphodiester bond
- (c)
To prevent unwanted self ligation
- (d)
To remove the phosphate group from the 3' end of a DNA molecule
For cloning the vector must have preferably
- (a)
Single recognition site
- (b)
A few recognition sites
- (c)
Many recognition sites
- (d)
Antibiotic resistance site
Vector for cloning genes in plants and animals is
- (a)
M13
- (b)
Ti plasmid
- (c)
Lambda phage
- (d)
Baculoviruses
The introduction of T DNA into plants involves
- (a)
Altering the pH of the soil, then heat shocking the plants
- (b)
Exposing the plants to cold for a brief period
- (c)
Allowing the plant roots to stand in water
- (d)
Infection of the plant by Agarobacterium tumefaciens
The antibiotic resistance gene method is tedious because.
- (a)
It involves plating of colonies in two media
- (b)
It rs not easy to identify the transformants easily
- (c)
It requires special markers
- (d)
It involves the continuous monitoring of the colony
Humulin a substance allied to insulin has been produced by introducing a coded DNA into the body of bacteria and this achievement is through
- (a)
Cloning
- (b)
Inoculation
- (c)
Hybridization
- (d)
Genetic engineering
Gel electrophoresis is used for
- (a)
Cutting DNA into fragments
- (b)
Separation of DNA fragments according to their size
- (c)
Construction of recombinant DNA by joining with cloning vectors
- (d)
Isolation of DNA molecules
International centre for genetic engineering and biotechnology established at
- (a)
New Delhi
- (b)
USA
- (c)
Italy
- (d)
Japan
PCR stands for
- (a)
Primary chain reaction
- (b)
Polymer chromosomal reaction
- (c)
Polymerase chain reaction
- (d)
Programmed chain reaction
Introduction of foreign genes for improving genotype is called
- (a)
Biotechnology
- (b)
Tissue culture
- (c)
Genetic engineering
- (d)
Both (1) & (3)
Which of the following bonds are formed by action of DNA ligase?
- (a)
Sugar-phosphate bond
- (b)
Phosphodiester bond
- (c)
Phosphate-phoshphate bond
- (d)
Both (1) & (2)
Genetic material of Retroviruses is
- (a)
DNA
- (b)
RNA
- (c)
Protein
- (d)
ssDNA
Boliver and Rodriguez developed
- (a)
Shuttle vector
- (b)
pBR322
- (c)
pUC19
- (d)
Both (1) & (2)
Shuttle vectors are not exemplified by
- (a)
yEP
- (b)
Ti plasmid
- (c)
BAC
- (d)
Both (2) & (3)
Addition of antibiotics in chemical engineering process helps in
- (a)
Mixing and aeration of media
- (b)
Maintaining anti-foaming conditions
- (c)
Maintaining aseptic conditions
- (d)
Maintenance of constant temperature and pH
Which of the following explains the role of heat shock during transformation?
- (a)
Binding of DNA to bacterial cell wall
- (b)
Uptake of DNA through transient pores
- (c)
Decreased efficiency of transformation
- (d)
All of these
Which of the following are not required while performing a transformation experiment?
- (a)
Insert DNA
- (b)
Ice cold calcium chloride
- (c)
900C
- (d)
Bacterial cells
The ability to transform normal cells into cancerous cells is found in
- (a)
Ti plasmid
- (b)
Retrovirus
- (c)
All plasmids
- (d)
Both (1) & (2)
Which of the following steps is/are catalysed by Taq polymerase in a PCR?
- (a)
Denaturation of template DNA
- (b)
Annealing of primers to template DNA
- (c)
Extension of primer end on template DNA
- (d)
All of these
Separation and purification by filtration, centrifugation of desired compound produced in bioreactor is a part of
- (a)
Downstream processing only
- (b)
Scaling up and downstream processing
- (c)
Upstream processing
- (d)
Screening for recombinants and downstream processing
Although the Ti plasmid has revolutionised plant genetic engineering one limitation of its use is that it
- (a)
Cannot infect broad leaf plants
- (b)
Cannot be used on fruit bearing plants
- (c)
Cannot transmit prokaryotic genes
- (d)
Does not infect cereal plants such as corn and rice
Which of the following is not a method of introducing alien DNA into host cells?
- (a)
Micro injection
- (b)
Heat shock method
- (c)
Being placed along with the cell into a gene gun
- (d)
Gel electrophoresis
Which of these are most widely used in genetic engineering?
- (a)
Plastid
- (b)
Plasmid
- (c)
Mitochondria
- (d)
Ribosome
An antibiotic resistance gene in a vector helps in selection of
- (a)
Competent cells
- (b)
Transformed cells
- (c)
Recombinant cells
- (d)
Both (2)&(3)
Given below is a sample of a protein DNA strand giving the base sequence on the opposite strands. What is so special shown in it?
5'_____________GAATTC____________3'
3'_____________CTTAAG____________5'
- (a)
Palindromic sequence of base pairs
- (b)
Replication completed
- (c)
Deletion mutation
- (d)
Start codon at the 5' end