Biotechnology : Principles and Processes
Exam Duration: 45 Mins Total Questions : 30
Which one is related to genetic engineering?
- (a)
Mutation
- (b)
Plasmid
- (c)
Plastid
- (d)
Heterosis
Plasmid is
- (a)
Fragment of DNA which acts as vector
- (b)
A fragment which joins two genes
- (c)
mRNA which acts as carrier
- (d)
An autotrophic fragment
The purpose .of the presence of restriction enzymes is
- (a)
Restricting the multiplication of the bacteria
- (b)
Restricting the multiplication of the virus
- (c)
Restricting the growth of the bacteriophage in bacterium
- (d)
Restricting the growth and multiplication of bacterium
Vector for cloning genes in plants and animals is
- (a)
M13
- (b)
Ti plasmid
- (c)
Lambda phage
- (d)
Baculoviruses
If the foreign DNA is inserted within tetR gene in the plasmid,
- (a)
The transformants will grow on the medium containing tetracycline
- (b)
The transformants will grow on the medium containing ampicillin
- (c)
The transformants will grow on the medium containing both tetracycline and ampicillin
- (d)
The transformants will not grow on the medium containing both
Which one is known as natural genetic engineer?
- (a)
Escherichia coli
- (b)
Salmonella sp
- (c)
Haemophilus sp
- (d)
Agrobacterium
Biolistic missile technique is used for
- (a)
Cloning the vector
- (b)
introduction of foreign DNA into plasmid
- (c)
Introduction of foreign DNA into host cells
- (d)
For transformation
If any protein encoding gene is expressed in a heterologus host it is known as
- (a)
Transformed protein
- (b)
Modified protein
- (c)
Heterologus protein
- (d)
Recombinant protein
Humulin a substance allied to insulin has been produced by introducing a coded DNA into the body of bacteria and this achievement is through
- (a)
Cloning
- (b)
Inoculation
- (c)
Hybridization
- (d)
Genetic engineering
Gel electrophoresis is used for
- (a)
Cutting DNA into fragments
- (b)
Separation of DNA fragments according to their size
- (c)
Construction of recombinant DNA by joining with cloning vectors
- (d)
Isolation of DNA molecules
Reporter enzyme is
- (a)
Used to join alien DNA
- (b)
Used to monitor transformation of host cells
- (c)
Used to cleave the desired DNA
- (d)
A gene that allows transformation
The specific DNA sequence where EcoRI cuts is
- (a)
GATTCG
- (b)
GAATTC
- (c)
GTTCAA
- (d)
TTCCAA
If gene of interest was inserted at Sal I site in pBR322 the resulting plasmid will confer resistance to
- (a)
Ampicillin
- (b)
Tetracycline
- (c)
Kanamycin
- (d)
Both (1) & (3)
In RDT, the term vector refers to
- (a)
Plasmids that can transfer foreign DNA into a living cell
- (b)
Plasmids that can cut DNA at specific bases
- (c)
Plasmids that can join DNA at specific bases
- (d)
Plasmids that can degrade harmful proteins
Shuttle vectors are not exemplified by
- (a)
yEP
- (b)
Ti plasmid
- (c)
BAC
- (d)
Both (2) & (3)
Genes for which of the following can serve as selectable marker?
- (a)
β-galactosidase
- (b)
Ampicillin
- (c)
Tetracycline
- (d)
All of these
Which of the following enzymes has been incorrectly matched with their function?
- (a)
Ligase-Molecular glue
- (b)
Endonuclease-Chemical Scalpel
- (c)
DNA polymerase-Joins nucleosides
- (d)
RNA polymerase-Joins nucleotides
Method involving entry of high velocity gold microparticles coated with DNA into plant cell is
- (a)
Protoplast fusion
- (b)
Transfection
- (c)
Biolistics
- (d)
Magic bullet action
Which of these are most widely used in genetic engineering?
- (a)
Plastid
- (b)
Plasmid
- (c)
Mitochondria
- (d)
Ribosome
Which of the following is not a basis of difference between plasmid and chromosomal DNA?
a. Presence of histones
b. Nature of histones
c. Nature of nucleotides
d. Linear form of genetic material
- (a)
b&c only
- (b)
a&d only
- (c)
a,b&d only
- (d)
a,b,c&d
Method in which recombinant DNA is directly injected into the nucleus of animal cell by using microneedles is called as
- (a)
Gene gun method
- (b)
Biolistic method
- (c)
Microinjection method
- (d)
Indirect method
The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of
- (a)
Insertional inactivation of alpha-galactosidase in non-recombinant bacteria
- (b)
Insertional inactivation of alpha-galactosidase in recombinant bacteria
- (c)
Inactivation of galactosidase enzyme in recombinant bacteria
- (d)
Non-recombinant bacteria containing beta-galactosidase
Which one of the following techniques made it possible to genetically engineer living organisms?
- (a)
Heavier isotope labelling
- (b)
Hybridization
- (c)
Recombinant DNA techniques
- (d)
X-ray diffraction
Which one of the following palindromic base sequences in DNA can be easily cut by EcoRI?
- (a)
5'______________CACGTA__________3'
3'______________CTCAGT__________5' - (b)
5'_____________CGTTCG_____________3'
3'_____________ATGGTA______________5' - (c)
5'____________GATATG_____________3'
3'____________CTACTA_____________5' - (d)
5'__________GAATTC____________3'
3'__________CTTAAG____________5'
Restriction endonucleases are enzymes which
- (a)
Remove nucleotides from the ends of the DNA molecule
- (b)
Make cuts at specific positions within the DNA molecule
- (c)
Recognize a specific nucleotide sequence for binding of DNA ligase
- (d)
Restrict the action of the enzyme DNA polymerase
In genetic engineering, a DNA segment (gene) of interest, is transferred to the host cell through a vector. Consider the following four agents (A-D) in this regard and select the correct option about which one or more of these can be used as a vector/vectors
Statements
(A) A bacterium (B) Plasmid
(C) Plasmodium (D) Bacteriophage
- (a)
(A), (B) & (D) only
- (b)
(A) only
- (c)
(A) & (C) only
- (d)
(B) & (D) only
Which one of the following hydrolyses internal phosphodiester bonds in polynucleotide chain?
- (a)
Lipase
- (b)
Exonuclease
- (c)
Endonuclease
- (d)
Protease
There are three basic steps in genetically modifying an organism. Which of the following is wrong statement about the process?
- (a)
Identification of the DNA with desirable genes
- (b)
Cleaving DNA segments with 'ligase' and joining them with endonuclease
- (c)
Introduction of the identified DNA into the host
- (d)
Maintenance of introduced DNA in the host and transfer of DNA to its progeny
in genetic engineering, the antibiotic resistant genes are used
- (a)
As selectable markers
- (b)
To select healthy vectors
- (c)
As sequences from where replication starts
- (d)
To keep the cultures free of infection
cDNA is
- (a)
Formed by reverse transcriptase
- (b)
Cloned DNA
- (c)
Circular DNA
- (d)
Recombinant DNA