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NEET Biology - Biotechnology : Principles and Processes Sample Question Paper

NEET Biology - Biotechnology : Principles and Processes

Exam Duration: 60 Mins Total Questions : 30

1 )

Producing a giant mouse in the laboratory was possible through

(a)
Gene mutation
(b)
Gene manipulation
(c)
Gene synthesis
(d)
Gene duplication

2 )

The plasmids are the

(a)
The enzymes present in the prokaryotes
(b)
Lysozymes in eukaryotes
(c)
Extra chromosomal DNA in bacteria
(d)
Mitochondrial DNA

3 )

The purpose of the presence of restriction enzymes in bacteria is

(a)
Gene cloning
(b)
Defence against infecting virus
(c)
Gene transfer
(d)
Genetic recombination

4 )

Match the following

	Column 1		Column 2
1.	Recombinant DNA	a.	Agaros gel
2.	Restriction enzyme.	b.	Cohen and boyer.
3.	PCR	c.	Linn and Arber
4.	Electrophoresis	d.	Kary Mullis

(a)
1d ; 2c ; 3b ; 4a
(b)
1d ; 2c ; 3a ; 4b
(c)
1c ; 2d ; 3b ; 4a
(d)
1b ; 2a ; 3d ; 4c

5 )

The first recombinant DNA was constructed by

(a)
Kary Mullis from Salmonella
(b)
Kary Mullis from Escherichia
(c)
Cohen & Boyer from Salmonella
(d)
Cohen & Boyer from Escherichia

6 )

Consider the following statements.
i. Many.restriction enzymes cleave both strands of DNA exactly at the same nucleotide position.
ii. When both strands of DNA is cleaved in the centre of the recognition sequence, it results in a blunt end.
iii. When both strands of DNA is cleaved in the centre of the recognition sequence, it results in a sticky end.

(a)
i & ii correct
(b)
i & iii correct
(c)
ii & iii correct
(d)
All correct

7 )

The process by which a piece of DNA is introduced in a host bacterium is

(a)
Transduction
(b)
Transfer
(c)
Transformation
(d)
Translocation

8 )

Vector for cloning genes in plants and animals is

(a)
M13
(b)
Ti plasmid
(c)
Lambda phage
(d)
Baculoviruses

9 )

If any protein encoding gene is expressed in a heterologus host it is known as

(a)
Transformed protein
(b)
Modified protein
(c)
Heterologus protein
(d)
Recombinant protein

10 )

An alien ~NA cannot become part of a chromosome anywhere along its length and replicate normally because

(a)
It requires a specific enzyme
(b)
The ligating enzyme is absent
(c)
It heeds an ori site for replication
(d)
The restriction enzyme digests them

11 )

Chemical knives of molecular biology are

(a)
Restriction endonucleases
(b)
Exonuclease
(c)
Reverse transcriptase
(d)
Ligase

12 )

Characteristics of vector include all, except

(a)
Presence of 'ori'
(b)
Presence of antibiotic resistance gene as selection marker
(c)
Large size
(d)
MCS

13 )

Name the organism which is the source of heat-stable PCR enzyme?

(a)
Thermophilus aquaticus
(b)
Haemophilus influenzae
(c)
Thermo proteus
(d)
Bacillus thuringiensis

14 )

Which of the following is not correct for a successful PCR?

(a)
Thermostable DNA polymerase
(b)
Nanogram of DNA template
(c)
Primer with exposed 5-OH group only
(d)
Deoxyribonucleoside triphosphates

15 )

Credit for construction of first recombinant DNA may be given to

(a)
Charles Darwin and Alfred Boyer
(b)
Stanley Cohen and Herbert Boyer
(c)
Messelson and Stahi
(d)
Esther and Joshua Lederberg

16 )

Which of the following enzymes has been incorrectly matched with their function?

(a)
Ligase-Molecular glue
(b)
Endonuclease-Chemical Scalpel
(c)
DNA polymerase-Joins nucleosides
(d)
RNA polymerase-Joins nucleotides

17 )

Which of the following statements doe not hold true for restriction enzyme?

(a)
It recognises a palindromic nucleotide sequence
(b)
It is an endonuclease
(c)
It is isolated from bacteriophages
(d)
It produces the same kind of sticky ends in different DNA molecules

18 )

Although the Ti plasmid has revolutionised plant genetic engineering one limitation of its use is that it

(a)
Cannot infect broad leaf plants
(b)
Cannot be used on fruit bearing plants
(c)
Cannot transmit prokaryotic genes
(d)
Does not infect cereal plants such as corn and rice

19 )

A plasmid

(a)
Shows dependent assortment
(b)
Has ability to replicate within bacterial cells independent of the control of chromosomal DNA
(c)
Cannot replicate
(d)
Contains genes for vital activities

20 )

Which enzyme is required to prevent unwanted self-ligation of vector DNA molecules in recombinant DNA technology?

(a)
DNA polymerase
(b)
DNA ligase
(c)
Alkaline phosphate
(d)
Reverse transcriptase

21 )

A set of bacterial clones, each containing a plasmid or phage, is called

(a)
Gene library
(b)
Gene pool
(c)
Genophore
(d)
Genome

22 )

Which of these are most widely used in genetic engineering?

(a)
Plastid
(b)
Plasmid
(c)
Mitochondria
(d)
Ribosome

23 )

Which of the following is not a basis of difference between plasmid and chromosomal DNA?
a. Presence of histones
b. Nature of histones
c. Nature of nucleotides
d. Linear form of genetic material