Biology - Biotechnology: Principles and Processes
Exam Duration: 45 Mins Total Questions : 30
Recombinant DNA is obtained by cleaving the pro DNA by
- (a)
Restriction endonucleases
- (b)
Exonucleases
- (c)
Ligases
- (d)
Primase
Genetic engineering is possible, because
- (a)
The phenomenon of transduction in bacteria is well understood
- (b)
We can see the DNA by electron microscope
- (c)
We can cut DNA at specific sites by endonqcleases like DNAs (1)
- (d)
Restriction endonucleases purified from bacteria can be used in vitro
Which one is related to genetic engineering?
- (a)
Mutation
- (b)
Plasmid
- (c)
Plastid
- (d)
Heterosis
Production of a human protein in bacteria by genetic engineering is possible because
- (a)
Bacterial cell can carry out the RNA splicing reactions
- (b)
The human chromosome can replicate in bacterial cell
- (c)
The mechanism of gene regulation is identical in humans and bacteria
- (d)
The genetic code is universal
The purpose of the presence of restriction enzymes in bacteria is
- (a)
Gene cloning
- (b)
Defence against infecting virus
- (c)
Gene transfer
- (d)
Genetic recombination
Which one is a correct statement?
- (a)
Restriction enzymes belong to a larger class of enzymes called nucleases
- (b)
The restriction enzymes are of three types
- (c)
Exo nucleases remove nucleotides from the middle of the DNA
- (d)
Endo nucleases are the joining enzymes
The function restriction modification system of the restriction enzymes
- (a)
These enzymes restrict the propagation of any foreign DNA in a bacterium
- (b)
These enzymes restrict the propagation of only viral DNA in a bacterium
- (c)
These enzymes restrict the propagation of bacterial DNA in a virus
- (d)
These enzymes restrict the propagation of viral DNA in a virus
How is a bacterium protected against the action of restriction enzymes?
- (a)
The bacterial DNA are resistant to the restriction enzymes
- (b)
The bacterial DNA is methylated within the recognition sequences
- (c)
The bacterial DNA are capped with methyl group
- (d)
The base sequences of bacteria are not recognized by restriction enzyms
Palindromes are the
- (a)
Methylated base sequences
- (b)
The formylated base sequences
- (c)
The base pair sequences that reads same on the two strands when orientation of reading is kept the same
- (d)
The base pair sequences that reads different on the two strands when orientation of reading is kept the same
The separation of DNA molecules is dependent on the
- (a)
Electric charges they carry
- (b)
Pore size of the agarose gel
- (c)
Both 1 and (2)
- (d)
Gravitational force
Ti plasmid is present in
- (a)
Escherichia coli
- (b)
Salmonella sp
- (c)
Agrobacterium sp
- (d)
Haemophilus sp
Which one is known as natural genetic engineer?
- (a)
Escherichia coli
- (b)
Salmonella sp
- (c)
Haemophilus sp
- (d)
Agrobacterium
The sequence of achieving the competent host is
- (a)
Treatment of bacteria with calcium chloride \(\rightarrow\) incubation of the cells with recombinant DNA on ice \(\rightarrow\) heat shock treatment \(\rightarrow\) putting back on ice
- (b)
Treatment of bacteria with sodium chloride \(\rightarrow\) incubation of the cells with recombinant DNA on ice \(\rightarrow\) heat shock treatment \(\rightarrow\) putting back on ice
- (c)
Incubation of the cells with recombinant DNA on ice \(\rightarrow\) treatment of bacteria with calcium chloride \(\rightarrow\) heat shock treatment \(\rightarrow\) putting back on ice
- (d)
ncubation of the cells with recombinant DNA on ice \(\rightarrow\) heat shock treatment \(\rightarrow\)treatment of bacteria with calcium chloride \(\rightarrow\) putting back on ice
The first human hormone produced by recombinant DNA technology is
- (a)
Thyroxin
- (b)
Progesterone
- (c)
Insulin
- (d)
Estrogen
Which vector can clone only a small fragment of DNA?
- (a)
Plasmid
- (b)
Cosmid
- (c)
BAC
- (d)
YAC
Taq polymerase is isolated from
- (a)
Escherichia
- (b)
Thermus aquaticus
- (c)
Haemophilus
- (d)
Salmonella
Which one is not the character of plasmid DNA?
- (a)
A circular extra chromosomal DNA
- (b)
Associated with Histones
- (c)
Contains very few genes but may not be necessary for the cell
- (d)
replicates independently
Chemical knives of molecular biology are
- (a)
Restriction endonucleases
- (b)
Exonuclease
- (c)
Reverse transcriptase
- (d)
Ligase
Who is given the credit for constructing first artificial recombinant molecule?
- (a)
Hargobind Khorana
- (b)
Stanley Cohen and Herbert Boyer
- (c)
Linus Pauling
- (d)
Arber and Nathans
Tumor including plasmid transforms
- (a)
Nematodes
- (b)
Bacteria
- (c)
Fungi
- (d)
Several dicot plants
The most commonly used bioreactor is of stirring type. The stirrer facilitates
- (a)
Temperature control
- (b)
pH control
- (c)
Oxygen availability
- (d)
Product removal
Which of the following enzymes has been incorrectly matched with their function?
- (a)
Ligase-Molecular glue
- (b)
Endonuclease-Chemical Scalpel
- (c)
DNA polymerase-Joins nucleosides
- (d)
RNA polymerase-Joins nucleotides
The ability to transform normal cells into cancerous cells is found in
- (a)
Ti plasmid
- (b)
Retrovirus
- (c)
All plasmids
- (d)
Both (1) & (2)
Which of the following steps is/are catalysed by Taq polymerase in a PCR?
- (a)
Denaturation of template DNA
- (b)
Annealing of primers to template DNA
- (c)
Extension of primer end on template DNA
- (d)
All of these
Stirrer in Stirred tank type bioreactor facilitates
- (a)
Oxygen delivery from outside to inside
- (b)
Mixing and aeration
- (c)
Temperature control
- (d)
Foam control
During the process of isolation of DNA, chilled ethanol is added to
- (a)
Remove proteins such as histones
- (b)
Precipitate DNA
- (c)
Break open the cell to release DNA
- (d)
Facilitate action of restriction enzymes
Which of the following is not a basis of difference between plasmid and chromosomal DNA?
a. Presence of histones
b. Nature of histones
c. Nature of nucleotides
d. Linear form of genetic material
- (a)
b&c only
- (b)
a&d only
- (c)
a,b&d only
- (d)
a,b,c&d
An antibiotic resistance gene in a vector helps in selection of
- (a)
Competent cells
- (b)
Transformed cells
- (c)
Recombinant cells
- (d)
Both (2)&(3)
Stirred-tank bioreactors have been designed for
- (a)
Availability of oxygen throughout the process
- (b)
Addition of preservatives to the product
- (c)
Purification of the product
- (d)
Ensuring anaerobic conditions in the culture vessel
Which of the following is considered as molecular glue?
- (a)
Alkaline phosphate
- (b)
Restriction endonuclease
- (c)
DNA ligase
- (d)
DNA polymerase