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OTI365 - NEET 2022
NEET Biology - Biotechnology: Principles & Processes Sample Questions

Biology - Biotechnology: Principles & Processes Question Paper 3

Exam Duration: 45 Mins Total Questions : 30

1 )

The term 'molecular scissors' refers to

(a)
recombinant DNA
(b)
restriction enzymes
(c)
Taq polymerase
(d)
palindromic nucleotide sequences.

2 )

The term 'chemical knife' refers to

(a)
polymerases
(b)
endonucleases
(c)
endonucleases
(d)
cellulases

3 )

The term 'recombinant DNA' refers to

(a)
DNA of the host cell
(b)
DNA with a piece of foreign DNA
(c)
DNA with selectable marker
(d)
DNA with more than one recognition sites

4 )

Which of the following contains the key tools for recombinant DNA technology?
(i) Restriction endonucleases, ligases, vectors
(ii) Ligases, host organism, polymerase enzymes
(iii) Vectors, Taq polymerase, primers
(iv) Restriction exonucleases, ligases, primers, bioreactors

(a)
(i), (ii) and (iii)
(b)
(i) and (ii)
(c)
(i), (iii) and (iv)
(d)
(iii) and (iv)

5 )

Read the given statements and select the correct option.
Statement 1 : The cloning vector is required to have very few, preferably single, recognition sites for the commonly used restriction enzymes.
Statement 2 : Presence of more than one recognition sites within a cloning vector will generate several fragments, which will complicate the process of gene cloning.

(a)
Both statements 1 and 2 are correct.
(b)
Statement 1 is correct but statement 2 is incorrect.
(c)
Statement 1 is incorrect but statement 2 is correct.
(d)
Both statements 1 and 2 are incorrect.

6 )

An advantage of using yeasts rather than bacteria as recipient cells for the recombinant DNA of eukaryotes is that yeasts can

(a)
produce restriction enzymes
(b)
excise introns from the RNA transcript
(c)
remove methyl groups
(d)
reproduce more rapidly.

7 )

Match the terms given in column I with their definitions in column II and select the correct anwser from codes given below.

	Column I		Column II
A	Transformation	i	Sequences cut by restriction enzymes
B	Recognition site	ii	Process by which DNA fragments are separated based on their size
C	Gel electrophoresis	iii	Plasmid DNA that has incorporated human DNA
D	Recombinant DNA	iv	Process by which bacteria take up pieces of DNA from the environment

(a)
A-(iii), B-(i), C-(ii), D-(iv)
(b)
A-(iv), B-(i), C-(ii), D-(iii)
(c)
A-(i), B-(ii), C-(iii), D-(iv)
(d)
A-(ii), B-(iii), C-(iv), D-(i)

8 )

The correct sequence of different steps of polymerase chain reaction is

(a)
annealing \(\rightarrow\) denaturation \(\rightarrow\) extension
(b)
denaturation \(\rightarrow\) extension \(\rightarrow\) annealing
(c)
denaturation \(\rightarrow\) annealing \(\rightarrow\) extension
(d)
extension \(\rightarrow\) denaturation \(\rightarrow\) annealing.

9 )

Given table gives an account of differences between PCR and gene cloning. Which of the following points shows the incorrect difference?

	Parameter	PCR	Gene cloning
1.	Efficient	More	Less
2.	Apparatus Requirement	DNA	Restriction enzyme, ligase, vector, bacterial cell
3.	Manipulation	in vitro	in vitro and in vivo
4.	Cost	More	Less
5.	Automation	Yes	No
6.	Error probability	Less	More
7.	Time for a typical experiment	2-4 days	4 hours
8.	Application	More	Less

(a)
1 and 3
(b)
4, 5 and 6
(c)
4 and 7
(d)
4, 7 and 8

10 )

Read the following statements and select the incorrect ones.
(i) When the transformed cells on agar plates containing ampicillin are spread, both transformed and untransformed cells will grow.
(ii) Restriction enzymes are used in isolation and separation of DNA from other macromolecules.
(iii) Downstream processing is one of the steps of rDNA technology.
(iv) Disarmed pathogen vectors are also used in transfer of rDNA into the host.

(a)
(ii) and (iii)
(b)
(iii) and (iv)
(c)
(i) and (iii)
(d)
(i) and (ii)

11 )

In agarose gel electrophoresis, DNA molecules are separated on the basis of their

(a)
separated on the basis of their
(b)
size only
(c)
charge to size ratio
(d)
all of the above.

12 )

Which of the following is not a source of restriction endonuclease?

(a)
Haemophilus influenzae
(b)
Escherichia coli
(c)
All of these
(d)
Bacillus amyloliquifaciens

13 )

Assertion: Genetic engineering can overcome the drawbacks of traditional hybridisation.
Reason: Genetic engineering can create desired DNA sequences to meet specific requirements.

(a)
If both assertion and reason are true and reason is the correct explanation of assertion.
(b)
If both assertion and reason are true but reason is not the correct explanation of assertion.
(c)
If assertion is true but reason is false.
(d)
If both assertion and reason are false.

14 )

Assertion : Use of chitinase enzyme is necessary for isolation of DNA from yeast cells but not in case of Spirogyra.
Reason: Fungal cell wall is made up of fungal cellulose or chitin.

(a)
If both assertion and reason are true and reason is the correct explanation of assertion.
(b)
If both assertion and reason are true and reason is the correct explanation of assertion.
(c)
If assertion is true but reason is false.
(d)
If both assertion and reason are false.

15 )

Which of the following is the example of chemical scissors:

(a)
ECo - RI
(b)
Hind - III
(c)
Bam - I
(d)
All the above

16 )

Which of the following is the example of direct gene transfer:

(a)
Micronjection
(b)
Electroporation
(c)
Particle gun
(d)
All the above

17 )

How many copies of DNA sample are produced in PCR technique after 6- cycle:

(a)
4
(b)
32
(c)
6
(d)
16

18 )

Important objective of biotechnology in agriculture section is

(a)
To produce pest resistant varieties of plants
(b)
To increase the nitrogen contant
(c)
To decrease the seed number
(d)
To increase the Plant weight

19 )

The name of durg used in cancer treatment produced by biotechnology is

(a)
Interferon
(b)
[HGH] Human growth hormone
(c)
TSH
(d)
insulin

20 )

The prerequisites for biotechnological production of antibiotics is

(a)
To search an antibiotic producing microorganism
(b)
To isolate the antibiotic gene
(c)
To join antibiotic gene with E.coli plasmid
(d)
All of the above

21 )

Modern biotechnology consist:

(a)
Genetic enginearing
(b)
tissue culture
(c)
Microbiology
(d)
All the above

22 )

GEAC makes decision regarding

(a)
the validity of GM research
(b)
the safety of introducing GM organism for public services
(c)
the validity of biopatents
(d)
more than one options are correct

23 )

The process of RNA interference has been used in the development of plants resistant to:

(a)
Nematodes
(b)
Fungi
(c)
Viruses
(d)
Insects

24 )

Bacillus thuringiensis forms protein crystals which contain insecticidal protein.
This protein:

(a)
does not kill the carries bacterium which is itself resistant to this toxin
(b)
binds with epithelial cells of midgut of the insect pest ultimately killing it
(c)
is coded by several genes including the gene cry
(d)
is activated by acid pH of the forgut of the insect pest

25 )

Which one of the following technique is used to produce the GM crops?